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FUJIFILM wako | L-012化學(xué)發(fā)光探針

L-012化學(xué)發(fā)光探針

生化學(xué)用 for Biochemistry3116094040028274.png

規(guī)格含量 : 90.0+%（HPLC）

制造商 : FUJIFILM Wako Pure Chemical Corporation（富士膠片和光）

儲(chǔ)存條件：冷藏（干冰運(yùn)輸）

CAS RN? : 143556-24-5

分子式 : C13H8ClN4NaO2

分子量 : 310.67

◆基本信息

L-012 是一種高靈敏度的化學(xué)發(fā)光（CHL）探針，比魯米諾（Luminol）試劑更有效。L-012 與多種類型的活性氧反應(yīng)，這些活性氧來(lái)自人血液和口腔中激活的中性粒細(xì)胞或者大鼠腹膜腔。該產(chǎn)品可以用于有辣根過(guò)氧化物酶的ELISA 試劑盒，可提高反應(yīng)靈敏度。

結(jié)構(gòu)式

溶解及保存：蒸餾水溶解成20 mM/L（6.23 mg/mL）的水溶液，再用50 mM/L的Tris鹽酸緩沖液（pH 7.5）稀釋至最終濃度為500 μM/L（有細(xì)胞的反應(yīng)體系）。另外，在沒(méi)細(xì)胞參與的反應(yīng)體系中時(shí)，建議使用終濃度為5~50 μM/L。母液冷凍保存，使用時(shí)再稀釋。

◆物性信息

外觀	白色~黃色，結(jié)晶~粉末
溶解性	可溶于水。

產(chǎn)品編號(hào)	產(chǎn)品名稱	產(chǎn)品規(guī)格	產(chǎn)品等級(jí)
120-04891	L-012 L-012化學(xué)發(fā)光探針	100 mg	－

拜爾迪是FUJIFILM wako授權(quán)代理商，

更多產(chǎn)品請(qǐng)咨詢：

北京拜爾迪生物技術(shù)有限公司

咨詢熱線：010-62960866

Q&A

Q： L-012能滲入細(xì)胞嗎?

A：由于L-012對(duì)脂質(zhì)高度親和，它比luminol和lucigenin更能具有細(xì)胞滲透性。另一方面，ROS無(wú)限制地在胞外釋放。因此L-012被認(rèn)為也能

檢測(cè)胞外ROS。

Q： L-012的使用濃度如何?

A：這取決于應(yīng)用和條件。

[體外] 培養(yǎng)細(xì)胞（EoL-1細(xì)胞）： 500 ~ 800 μmol/L.

全血和分離的中性粒細(xì)胞： 400 μmol/L

多形核中性粒細(xì)胞（PMNs）（由小鼠中分離）： 2 mmol/L

無(wú)細(xì)胞的樣品： 4 μmol/L, 15 μmol/L, and 50 μmol/L

[體內(nèi)] Balb/c 小鼠[皮下]： 20 ~ 80 μL of L-012(5mg/mL)

Balb/c 小鼠[腹膜內(nèi)]： 100 μL of L-012 (15mg/mL)

C57BL/10.Q (B10.Q) 小鼠 [腹膜內(nèi)]： 1 ~ 75 mg/kg

Q：是否可用于動(dòng)物活體成像？

A：如果所用的小動(dòng)物成像儀中有發(fā)光檢測(cè)探頭，就可以進(jìn)行檢測(cè)。以下是文獻(xiàn)報(bào)道過(guò)的儀器型號(hào)：

?IVIS 100 imaging systems

  ?Infinite M200 [Tecan]
  ?Luminescence Reader BLR-201 [Aloka]
  ?Beckman-Coulter DTX880 multimode plate reader [Beckman]

Q：是否可用于固定細(xì)胞？

A：目前尚無(wú)證據(jù)表明，活性氧（ROS）在細(xì)胞活性停止后仍然存在。普遍看法是認(rèn)為在細(xì)胞活性停止后（細(xì)胞死亡）ROS消失。固定組織或

細(xì)胞會(huì)停止細(xì)胞活性，因此不建議用于ROS檢測(cè)。

本品無(wú)需固定檢測(cè)對(duì)象即可檢測(cè)到ROS，使用方法包括但不限于將其添加到細(xì)胞培養(yǎng)上清液中或進(jìn)行動(dòng)物腹腔注射。

參考文獻(xiàn)

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